ABSTRACT
Objective To investigate the preliminary clinical results of treating scaphoid fracture by percutaneous fixation with arthroscope assistance. Methods From October 2009 to May 2015, a consecutive series of 12 patients with scaphoid fracture were treated by percutaneous fixation with arthroscope assistance. Meanwhile TFCC was man-aged if necessary. As followed, X-ray was adopted for assessment bone healing at 6-month postoperation, 12-month postoperation. Postoperative evaluations included clinical measurement (grip strength and motion range), radiograph-ic, and functional (modified Mayo wrist score) parameters, Herbert and Fisher scaphoid fracture parameters. Healthy wrist as control group. Results All the scaphoid fracture were healed with an average healing time of 24 weeks. All 12 cases were followed for an average of 18.5 months. The function was rated excellent in 7 cases, good in 5 cases according to the modified Mayo wrist score. There was no difference between the injuried wrist and control group. Conclusion For scaphoid fracture, percutaneous fixation with arthroscope assistance is a reliable and minimally in-vasive method to treat scaphoid fracture.
ABSTRACT
Total knee replacement surgery is commonly used in end-stage diseases of the knee. It is important for improving surgical efficacy and patient satisfaction by promoting early rehabilitation of patients and improving knee function.
ABSTRACT
BACKGROUND: Whether bone morphogenetic protein 2 (BMP-2) can be transduced into marrow stromal cells (MSCs) and produce osteogenic effects by viral or non-viral vector remains unclear? OBJECTIVE: To observe the expression of cultured rabbit MSCs transfected with pcDNA3-hBMP2 in vitro. Simultaneously, the MSCs were transfected but not screened and then transplanted into autologous muscle to investigate the osteogenic capability by X-ray. DESIGN, TIME AND SETTING: A controlled observation experiment was performed at the Department of Orthopedics, Liaoning Medical University between November 2004 and April 2005. MATERIALS: Six adult New Zealand rabbits, of either gender, weighing 2.0-3.0 kg, were included for this study. BMP2 antibody was the product of Sanaka Company, USA. pcDNA3-hBMP2 was provided by Professor Pu Qin from the Department of Biochemistry, Fourth Military Medical University of Chinese PLA). Restriction enzyme was purchased from Takara biotechnology (Dalian) CO., LTD., China. METHODS: Super-purified plasmid pcDNA3-hBMP2 was extracted from E. coli. Bone marrow was taken from the adult rabbit femur for harvesting MSCs by density gradient separation. The MSCs were divided into the following 4 groups: Group A, cells were transfected and screened by G418; Group B, cells were transfected by pcDNA3-hBMP2; Group C, cells were transfected by empty vector pcDNA3; Group D, only transfection reagent Fugene 6 was added.MAIN OUTCOME MEASURES: Transient BMP2 expression was analyzed by immunohistochemistry. Expression of osteocalcin and collagen I was examined by immunohistochemistry and in situ hybridization, respectively. Two weeks after transfection, MSCs from the group B were autologously transplanted into the muscle. Four weeks later, X-ray assay was used to observe bone formation.RESULTS: pcDNA3-hBMP2 was successfully transduced into MSCs and transiently expressed BMP2 100%. Four weeks after gene transfection, expression levels of osteocalcin and collagen I were significantly higher in the group A than in the groups C and D. X-ray results demonstrated new bone formation four weeks after MSCs transplanted into the muscle.CONCLUSION: pcDNA3-hBMP2 can safely and efficiently transfect MSCs and induce them to differentiate towards osteoblasts by secreting BMP2.